메뉴 바로가기

본문 바로가기

홈 > 도서관안내 > 도서관 소개

도서관 소개

표지이미지
자료유형 : 연구보고[원문]
서명 / 저자 : BT기반 소나무재선충병 친환경 방제제 개발 (2016~2020) / 이상현, 고상현, 김준헌, 이선근, 김동수, 김창진, 김진철, 김재수
개인저자 : 이상현
고상현
김준헌
이선근
김동수
김창진
김진철
김재수
발행사항 : 서울 : 국립산림과학원, 2021.
형태사항 : >275 p. : 도표 ; 26 cm
총서사항 : 연구보고 ; 21-17
ISBN : 9791160193817
청구기호 : 634.9072 오73 21-17
QR Code 정보
BT기반 소나무재선충병 친환경 방제제 개발 (2016~2020) QR Code
 

소장자료

* 대출된 도서만 예약가능


관계정보

부가정보

요약 ························································································································1
1. 서론 ···················································································································9
2. 본론 (연구결과) ······························································································13
가. 곤충생장제어물질을 이용한 매개충 방제제 개발 ···············································15
(1) 소나무재선충병 매개충에 대한 유충호르몬 대항물질 탐색 ···································15
(가) 솔수염하늘소의 유전자 클로닝 ········································································15
(나) 해당 유전자 도입과 발현 ··················································································16
(2) 곤충별 살충활성 평가체계 구축 ···········································································17
나. 식물내생미생물 활성물질 기반 살재선충제 개발 ···············································19
(1) 소나무류 내생미생물 유래 물질을 이용한 살재선충제 개발 ·································19
(가) 소나무류에서 분리된 내생 미생물 ····································································19
(나) 분리된 내생미생물의 동정 ···············································································19
(다) 내생미생물 유래 물질에서 살재선충 효능 검정 ················································20
(라) 살재선충 물질의 구조 동정 및 살재선충 효능 검정 ··········································21
(마) R. ornithinolytica 의 추출물질의 소나무 약해 검정 ········································24
(2) 내생세균 생물변환을 통한 살재선충 효과 ····························································25
(가) 식물내생세균 수집 및 분리 ··············································································25
(나) 식물내생세균 배양액, S. avermitilis 의 배양체 추출물, Abamectin의
살재선충 효과 ··································································································26
(다) 식물내생세균의 추출물 및 생물전환한 식물내생균 배양추출물의
살재선충 효과 ··································································································26
(라) 식물내생세균 단백질을 이용한 Abamectin의 생물전환 ·································28
(3) 선발 내생미생물 개량을 통한 살재선충 물질 생산 극대화 ····································29
(가) 소나무재선충 살재선충 활성물질 생산 미생물의 유전체 염기서열 분석 ···········29
(나) 살재선충 활성물질 생산 미생물의 2차 대사산물 생산 유전자 클러스터 분석 및
타 균주와의 비교 분석 ·····················································································31
(다) 살재선충 활성 대량 스크리닝 시스템 구축 ·······················································38
(라) 형질전환체의 재선충 활성 검정 ·······································································38
다. 방선균 유래 소나무재선충 방제제 개발 및 에버멕틴 이용 기술 개발 ·················44
(1) 살재선충 활성균주로부터 활성물질의 분리정제 및 구조분석 ·······························44
(가) 활성균주 선발 ··································································································44
(나) 활성물질 구조 동정 ··························································································48
(다) 활성물질 대량배양 ···························································································56
(라) 활성물질의 효능 평가 ······················································································64
(마) 유기농자재 등록 자료확보를 위한 안전성 평가 ················································71
(2) 고효율 에버멕틴 유도체 개발 ···············································································75
(가) LC-MS/MS를 이용한 에버멕틴 및 에버멕틴 유도체 정성/정량 분석 방법 ·····75
(나) 에버멕틴 개량 효소 발굴 ··················································································76
(다) 에버멕틴 유도체 구축 ······················································································78
(라) 에버멕틴 유도체 효능 검정 ··············································································88
(마) Avermectin 당화효소 개량 ············································································89
라. 유도저항성을 활용한 소나무재선충병 방제제 개발 ··········································101
(1) 살재선충제 단제 및 합제의 포장약효 지속성 조사시험 ······································101
(가) 살재선충제 단제 및 합제 제제 개발 ·······························································101
(나) 살재선충제 단제 및 합제 3종의 포장시험 ······················································105
(다) 선발된 살재선충제 단제 및 합제의 포장 지속성 검사 ····································108
(2) 유도저항성 소재를 이용한 소나무재선충병 방제 ···············································110
(가) 유도저항성 소재 선발 ····················································································110
(나) 유도저항성 소재의 최적 제제 선발 ································································117
(다) 선발한 유도저항성 제제의 최적 처리조건 확립 ··············································126
(3) 저항성 유도 우수 활성 미생물 선발 ···································································132
(가) 유도저항성 활성 검정을 통한 우수활성 미생물 선발 ······································132
(나) 우수활성 미생물 JCK-1398(BC)를 활용한 방제제 개발 ·····························137
(4) 유도저항성 소재 및 미생물을 이용한 항공살포용 소나무재선충병 방제제 개발 · 142
(가) 유도저항성 소재(ASM) 및 활성미생물(JCK-1398(BC))
항공살포용 제제 제조 ····················································································142
(나) ASM제제 및 JCK-1398(BC)제제의 항공살포 조건 확립을 위한
in vivo 검정 ··································································································144
(5) 유도저항성 소재 및 미생물을 이용한 항공살포용 소나무재선충병 방제제
실용화평가 ········································································································148
(6) 소나무의 전사체 분석 기반 통합적 네트워크 분석 ·············································149
(가) 유도저항성 소재 및 미생물이 처리된 소나무의 전사체 분석 ··························149
(나) 유도저항성 활성 소재 및 활성균주가 처리된 소나무의 전사체 분석 ··············160
(7) 유도저항성 소재 및 균주가 처리된 소나무 유묘의 근권에서의 미생물 군집분석 161
(가) 저항성 유도 소재(ASM과 MeSA)를 처리한 소나무 유묘의 근권
미생물군집 분석 ····························································································161
(나) 저항성 유도 미생물을 처리한 소나무 유묘의 근권 미생물 군집분석 ··············164
(8) 유도저항성 미생물 및 소재의 작용기작 구명 ·····················································168
(가) B. thuringiensis JCK-1233균주와 유도저항성 활성물질의 작용기작 구명 168
(나) 유도저항성 소재 ASM의 소나무재선충병 방제 작용기작 구명 ······················176
(다) 유도저항성 소재 MeSA의 소나무재선충병 방제 작용기작 구명 ····················178
마. 소나무재선충병 매개충 방제용 천연물 방제제 개발 ········································182
(1) 미생물 소재 확보 및 라이브러리 구축 ································································182
(가) 토양 시료 채집 및 균주 분리 ··········································································182
(나) 솔수염하늘소에 대한 병원성 진균 라이브러리 구축 ·······································186
(다) 솔수염하늘소에 방제 미생물 소재의 선발 ······················································187
바. 수의용 등록약제 적용기술 개발 ·······································································251
사. 유기농자재 적용기술 개발 ···············································································252
(1) 유기농자재의 독성 평과 ·····················································································252
(2) 에스터의 북방수염하늘소 살충 효과 ··································································252
(3) Terpenoid의 매개충 접촉독성 평가 ··································································253
아. 알킬옥시알콜을 이용한 방제제 개발 ································································254
(1) 알킬옥시알콜의 살재선충 효능 검정 ··································································254
(가) 알킬옥시알콜(=알킬옥시알칸올) 합성 ····························································254
(나) 알킬옥시알콜의 살재선충 효과검정 ······························································254
(2) 벤질옥시알콜의 살재선충 효능 검정 ··································································255
(3) 살재선충 활성 물질의 작용 기작 연구 ································································256
3. 참고문헌 ·······································································································259
4. 연구성과 ·······································································································269
가. 논문게재 ·········································································································271
나. 산업재산권 ···········································································································273
(1) 특허 출원 ···········································································································273
(2) 특허 등록 ···········································································································274
(3) 국제 특허 출원 ···································································································274