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자료유형 : 연구자료[원문]
서명 / 저자 : 표고버섯의 유전체 연구 - 산마루2호와 수향고 - / 유선화, 김명길, 가강현, 유림, 장영선, 최돈하, 이성숙, 이수연, 홍창영, 안지은 [공저]
개인저자 : 유선화
김명길
가강현
유림
장영선
최돈하
이성숙
이수연
홍창영
안지은
발행사항 : 서울 : 국립산림과학원, 2017
형태사항 : >177 p. : 삽화,도표 ; 26 cm
총서사항 : 연구자료 ; 제741호
서지주기 : 참고문헌 : p. 167-170
일반주기2 : 비매품
ISBN : 9791160191790
청구기호 : 634.9072 유54표 제741호
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표고버섯의 유전체 연구 - 산마루2호와 수향고 - QR Code
 

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Contents
Ⅰ. 유전체 연구의 이해와 표고버섯의 유전체 연구동향 ····························· 7
1. 유전체 분석 연구 ·························································································· 9
1) 서론 ················································································································· 9
2) 포스트 게놈 시대 ·························································································· 9
3) 유전체 해독 ··································································································· 11
2. 전사체 분석 ··································································································· 12
1) 서론 ················································································································ 12
2) 전사체 분석 방법 ························································································· 13
3. 표고버섯의 유전체 연구 동향 ···································································· 14
1) 서론 ················································································································ 14
2) 표고버섯의 유전체 해독 ············································································· 14
3) 표고버섯의 전사체 분석 ············································································· 15
4) 표고버섯의 유전체 연구의 활용 ································································ 17
5) 표고버섯의 유전체 정보 공개 ···································································· 18
Ⅱ. 산마루2호와 수향고의 전사체 분석 ························································ 21
1. 서론 ················································································································ 23
2. 연구 방법 ······································································································· 25
1) RNA 추출 및 QC ··························································································· 25
2) Illumina Hiseq2000을 이용한 염기서열 분석 ·········································· 25
3) Sequencing data 분석 ·················································································· 30
3. 연구 결과 ········································································································ 33
1) RNA QC ·········································································································· 33
2) RNA sequencing ··························································································· 35
3) De novo assembly ························································································ 39
4) Annotation ····································································································· 43
5) DEG(Differentially Expressed Genes) 분석 ················································ 56
6) Target gene analysis ···················································································· 135
Ⅲ. 표고버섯 수향고의 유전체 분석 ······························································· 141
1. 서론 ················································································································ 143
2. 연구 방법 및 결과 ························································································ 143
1) 수향고 genomic DNA 분리 ········································································ 143
2) Library제작 및 sequencing ········································································· 144
3) 표고버섯 수향고 유전체 de novo assembly ············································ 145
4) 수향고 유전체 annotation 결과 ································································· 159
IV. 참고문헌 ········································································································ 167